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大鼠全身经~(60)Co-γ线800rad一次照射后24小时,测定血浆、肝、脾和小肠粘膜中cAMP含量,结果表明:照射组和对照组两组血浆cAMP含量(43.82±7.68,41.26±6.71Pmol/ml血浆,P>0.05)和肝中cAMP含量(7.80±1.14,8.05±2.08Pmol/mg蛋白质,P>0.05)都无明显改变,脾cAMP含量照射组较对照组有显著增高(41.28±8.06,28.95±12.67Pmol/mg蛋白质,P<0.05),而小肠粘膜cAMP含量照射组较对照组则显著降低(4.89±2.53,7.87±2.02Pmol/mg蛋白质,P<0.01)。 3’,5’-环腺苷酸(cAMP)是激素的第二信使,在调节细胞代谢、细胞生化和生理等过程中有重要的作用。组织中环腺苷酸水平受内源和外源很多因素的影响,如激素、神经、免疫、物理、化学、电离辐射等因素所影响,电离辐射损伤会影响到组织细胞中cAMP含量和cAMP生成与分解的酶活性。近年来有报导动物γ-线一次全身照射后,肝、脾中cAMp含量有变化,一般认为肝是辐射较不敏感的组织,脾是辐射敏感组织。动物经照射后肝中cAMP含量变化不大,而脾则增高。但也有报导肝、脾中cAMP含量都增高。对于肠粘膜中cAMP含量变化报导甚少,但肠也是辐射敏感组织。因此,我们观察了大鼠经~(60)Co-γ线照射后,血浆、肝、脾、小肠粘膜等组织中cAMP含量的变化,以积累这方面的资料。
The cAMP content of plasma, liver, spleen and small intestine mucosa was measured 24 h after irradiation of ~ (60) Co-γ line of 800rad in whole body of rats. The results showed that the plasma cAMP content in the irradiated and control groups (43.82 ± 7.68, 41.26 ± 6.71Pmol / ml plasma, P> 0.05) and cAMP content in liver (7.80 ± 1.14,8.05 ± 2.08Pmol / mg protein, P> 0.05). There was no significant change in cAMP content in the irradiated group compared with the control group (41.28 ± 8.06,28.95 ± 12.67Pmol / mg protein, P <0.05). However, the cAMP content of the intestinal mucosa in the irradiation group was significantly lower than that in the control group (4.89 ± 2.53,7.87 ± 2.02Pmol / mg protein, P <0.01). 3 ’, 5’-CAMP is the second messenger of hormones and plays an important role in the regulation of cell metabolism, cell biochemistry and physiology. Adenosine levels in tissues are influenced by many factors, such as hormones, nerves, immunity, physics, chemistry, ionizing radiation, and other factors, ionizing radiation damage affects the cAMP content in tissue cells and cAMP production and Decomposition of the enzyme activity. In recent years there are reports of animal γ-ray after a whole body irradiation, the liver, spleen cAMp content changes are generally believed that the liver radiation is less sensitive to the tissue, spleen is radiation-sensitive tissue. After irradiation, the content of cAMP in the liver changed little, but the spleen increased. But there are also reports of liver, spleen, cAMP levels were increased. Little is known about changes in cAMP content in the intestinal mucosa, but the gut is also a radiation-sensitive tissue. Therefore, we observed the change of cAMP content in plasma, liver, spleen, small intestine mucosa and other tissues irradiated by ~ (60) Co-γ ray in order to accumulate this data.