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本文对主要组织相容性复合分子(MHC)在亚剂量链脲佐菌素(STZ)合并完全弗氏佐剂(CFA)诱发大鼠抗β-细胞的自身免疫应答中的作用进行了评价。 用同基因异系种(LEW.1A和LEW.1W),5～6周龄的雄性大鼠52只,体重117±27.9g,腹腔内注射0.5mlCFA,并在24小时后注射亚剂量STZ25mg/kg体重,隔周1次共3次。同时注射同剂量STZ的9只,CFA13只。在第二组实验中,LEW1A和1W雄鼠各15只仅注射CFA。各次注射CFA/STZ后7天分别杀死3或4只。CFA注射后7天分别检测外周血白细胞(WBC)及脾重量(脾重/体重×100)。分别在CFA和STZ注射前从不禁食的动物取血。用葡萄糖氧化酶法检测血糖浓度。按Zie-gler等的方法测定胰岛素含量,按Gottschling等
The role of major histocompatibility complex molecule (MHC) in autoantibody responses to anti-beta-cells in subcutaneous doses of streptozotocin (STZ) and complete Freund’s adjuvant (CFA) was evaluated in this study. Fifty-two male rats of 5 to 6 weeks old, weighing 117 ± 27.9 g, were injected intraperitoneally with 0.5 ml of CFA, and 24 hours later sub-doses of STZ 25 mg / kg body weight, every other week a total of 3 times. At the same time inject the same dose of STZ 9, CFA13 only. In the second set of experiments, 15 of the LEW1A and 1W male mice were injected with only CFA. Three or four mice were killed 7 days after each injection of CFA / STZ. Peripheral blood leukocytes (WBC) and spleen weights (spleen weight / body weight × 100) were measured 7 days after CFA injection. Blood was taken from animals that were not fasting before CFA and STZ injection, respectively. Glucose oxidase method for detecting blood glucose levels. Insulin content was measured by the method of Zie-gler et al., According to Gottschling et al