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目的分析UHPLC法测定柴黄片中柴胡皂苷a和柴胡皂苷d含量的方法及效果。方法取柴黄片中成药制剂,采用Hypersil C_(18)色谱柱法,以乙腈磷酸水溶液作为流动相,科学设定梯度洗脱值,控制流速,检验波长控制在210~254nm,柱温则为30~35℃。结果柴黄片中柴胡皂苷a线性范围为0.98~5.67μg(r~2=0.9991),柴胡皂苷d线性范围为0.059~0.052μg(r~2=0.9997);柴胡皂苷a的平均加样回收率为98.0%,柴胡皂苷d的平均加样回收率为96.5%,柴胡皂苷a和柴胡皂苷d的RSD分别为2.0%、1.8%。结论 UHPLC法不仅操作简便,同时安全可靠,具有良好的重复性,在柴黄片柴胡皂苷类物质含量测量中发挥重要作用,可为中成药方剂质量体系的完善提供更多依据。
Objective To analyze the method and effect of determination of saikosaponin a and saikosaponin d in Shixian tablet by UHPLC. Methods The traditional Chinese medicine preparation of Chaihuang tablet was obtained. Hypersil C_ (18) column method and acetonitrile - phosphoric acid aqueous solution were used as the mobile phase. The gradient elution was scientifically set and the flow rate was controlled. The wavelength was controlled at 210-254 nm and the column temperature was 30 ~ 35 ℃. Results The linear range of saikosaponin a was 0.98 ~ 5.67μg (r ~ 2 = 0.9991), the linear range of saikosaponin d was 0.059 ~ 0.052μg (r ~ 2 = 0.9997) The average recoveries of saikosaponin d were 96.5% and the RSDs of saikosaponin a and saikosaponin d were 2.0% and 1.8%, respectively. Conclusion The UHPLC method is not only easy to operate, but also safe and reliable with good reproducibility. It plays an important role in the determination of saikosaponin in Chaihuang tablets, which can provide more basis for the improvement of prescription quality system of Chinese patent medicines.