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目的探讨PTEN基因和ICAM-1与肝细胞发生、发展、侵袭转移的关系及临床意义。方法应用组织芯片技术和免疫组化SABC法对46例手术切除的经病理确诊为肝细胞癌变的标本进行实验研究。结果正常肝细胞组织中PTEN有4例呈强阳性表达,ICAM-1均呈阴性表达。肝细胞癌变患者PTEN基因阳性率为65.2%(30/46),而ICAM-1阳性率为58.7%(27/46),癌旁组织中PTEN基因和ICAM-1阳性率分别为90.9%(30/33),87.8%(29/33),两者表达与患者年龄、性别、肿瘤大小及AFP是否阳性无关,而与肝细胞癌变的病理分级,包膜及癌栓的有无关系密切。其中PTEN与HBsAg是否阳性无关,ICAM-1与HBsAg是否阳性关系密切。结论 PTEN基因具有抑制肝细胞的癌变发生、发展、侵袭转移的作用,而ICAM-1对肝细胞的癌变、发生、发展、侵袭转移具有促进作用。本研究发现两者在肝细胞的癌变、发生、发展、侵袭转移过程中两者呈负相关。
Objective To investigate the relationship between PTEN gene and ICAM-1 and the occurrence, development, invasion and metastasis of hepatocytes and its clinical significance. Methods Tissue microarray technique and immunohistochemical SABC method were used to study 46 cases of pathologically confirmed hepatocellular carcinoma (HCC). Results In normal liver tissue, 4 cases of PTEN were strongly positive and ICAM-1 was negative. The positive rate of PTEN gene in hepatocellular carcinoma was 65.2% (30/46), while the positive rate of ICAM-1 was 58.7% (27/46). The positive rates of PTEN gene and ICAM-1 in hepatocellular carcinoma were 90.9% (30 /33),87.8%(29/33), both expression and patient age, gender, tumor size and AFP is not related to the positive, but with the pathological grade of hepatocellular carcinoma, capsule and tumor embolism are closely related. Which PTEN positive and HBsAg has nothing to do, whether ICAM-1 and HBsAg positive are closely related. Conclusions PTEN gene can inhibit the occurrence, development, invasion and metastasis of hepatocellular carcinoma, and ICAM-1 can promote the carcinogenesis, development, invasion and metastasis of hepatocellular carcinoma. This study found that both of them have a negative correlation in carcinogenesis, development, invasion and metastasis of hepatocytes.