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目的:初步筛选小红参抗心肌缺血的活性部位。方法:采用pit尾静脉注射方法制备大鼠急性心肌缺血模型。将SD大鼠随机分为8组:正常对照组、模型组、消心痛组、复方丹参滴丸组、小红参A、B、C、D药物组。各组均在连续灌胃15d后造模,测定大鼠15、30s,1、3、5、10、20min心电图,腹主动脉采血,制备血清测定AST、CK、LDH、SOD、MDA含量。结果:小红参提取物各极性段物质A、B、C、D都具有抗心肌缺血的作用(J点位移与模型组比较P<0.01),以C段物质作用比较明显;小红参各极性段物质可明显降低血清心肌酶AST、CK、LDH和MDA的含量,提高血清SOD活性(P<0.05或P<0.01),作用以B、C两段物质较为明显。结论:小红参抗心肌缺血的活性部位主要集中在B、C段,作用可能与抗氧化有关,具体有效成分及作用机制还待进一步研究。
Objective: To screen the active site of anti-myocardial ischemia of red ginseng. Methods: Acute myocardial ischemia model was induced by pit tail vein injection. The SD rats were randomly divided into 8 groups: normal control group, model group, XiaoXiaTong group, compound Danshen dripping group, small red ginseng A, B, C, D drug group. The rats in each group were sacrificed 15 days after continuous gavage. The electrocardiogram, abdominal aorta blood samples were collected at 15, 30s, 1, 3, 5, 10, and 20 minutes after the rats were sacrificed. Serum levels of AST, CK, LDH, SOD and MDA were measured. Results: A, B, C, and D in each polar fraction of red ginseng extract had the function of anti-myocardial ischemia (J point displacement compared with model group, P <0.01) Participation of the polar substances can significantly reduce serum levels of myocardial enzymes AST, CK, LDH and MDA, increase serum SOD activity (P <0.05 or P <0.01), the role of B, C two more obvious substances. CONCLUSION: The active fractions of red ginseng against myocardial ischemia are mainly concentrated in the B and C segments. The effect may be related to the antioxidation. The specific active ingredients and mechanism of action are still to be further studied.