目的研究胃癌患者外周血单个核细胞（ＰＢＭＣ）由细菌内毒素（ＬＰＳ）诱导产生ＴＮＦα的能力及胃癌患者自身血浆和吲哚美辛对产生ＴＮＦα的影响．方法采用ＥＬＩＳＡ法测定３６例胃癌患者（男２７例，女９例，年龄３８岁～７１岁，平均５２岁；其中，Ⅰ、Ⅱ期１７例，Ⅲ、Ⅳ期１９例）；２０例良性胃病患者（男１３例，女７例，年龄３２岁～７５岁，平均４９岁）；及２０例正常人（男１２例，女８例，年龄２５岁～５２岁，平均４７岁）ＰＢＭＣ经ＬＰＳ诱导培养上清液中ＴＮＦα水平．胃癌患者ＰＢＭＣ又给予另外两种处理：胃癌自身血浆＋ＬＰＳ和吲哚美辛＋ＬＰＳ．结果ＴＮＦα水平（μｇ／Ｌ）胃癌组（３０４±１６６）较良性胃病组（１０３±０６２）与正常组（０７６±０５７）均显著升高（Ｐ＜００５），良性胃病组与正常组之间无显著差异（Ｐ＞００５）；胃癌患者的不同病理分型间、分期间亦无显著差异（Ｐ＞００５）．胃癌组ＰＢＭＣ经ＬＰＳ诱导加自身血浆处理则ＴＮＦα水平较仅用ＬＰＳ诱导者显著下降（２０７±０９４，Ｐ＜００５），而经吲哚美辛处理较仅用ＬＰＳ处理者显著升高（６４±３０６，Ｐ＜００１）．结论ＴＮＦα水平可作为胃癌诊断的潜在标? Objective To investigate the ability of peripheral blood mononuclear cells (PBMCs) from gastric cancer patients to induce TNFα production by bacterial endotoxin (LPS) and the effects of autologous plasma and indomethacin on TNFα production in gastric cancer patients. Methods Thirty-six patients with gastric cancer were detected by ELISA (27 males and 9 females, aged 38 to 71 years, mean 52 years; 17 of them were in stage I and II, 19 of stage III and IV); 20 cases of benign gastropathy Patients (13 males and 7 females, aged 32 to 75 years old, average 49 years old); and 20 normal subjects (12 males and 8 females, aged 25 to 52 years, mean 47 years) PBMCs via LPS Induced TNFα levels in the culture supernatant. Patients with gastric cancer PBMC were given two additional treatments: gastric autologous plasma + LPS and indomethacin + LPS. Results The level of TNFα (μg/L) in gastric cancer group (304±166) was significantly higher than that in benign gastropathy group (103±062) and normal group (076±057) (P <005), There was no significant difference between benign gastropathy group and normal group (P>005); There was no significant difference between different pathological types and periods of gastric cancer patients (P>005). In the gastric cancer group, PBMC induced by LPS and treated with autologous plasma had significantly lower levels of TNFα than those induced by LPS only (207±094, P<005), whereas treatment with indomethacin was more effective than LPS alone. Significantly increased (64±306, P<001). Conclusion The level of TNFα can be used as a potential marker for the diagnosis of gastric cancer.