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为探讨重庆市某社区人群内皮素1(Endothelin-1,ET-1)基因G8002A,+138A/-两个位点基因多态性与原发性高血压(Essential hypertension,EH)的相关性,采用PCR-RFLP和PCR-SSP方法分别检测95名EH患者及150名血压正常者组(NT)的G8002A和+138A/-基因多态性.结果发现:在EH组中发现+138A/-位点基因型与舒张压相关(p=0.005),其中4A4A型舒张压比3A3A型高13 mmHg(p=0.005),比3A4A型高12 mmHg(p=0.04).将G8002A与+138A/-位点联合分析后发现舒张压在EH病人中GA4A4A型较其他几型平均高达近20 mmHg,呈显著相关性(p<0.01).认为ET-1基因+138A/-的A插入型变异位点可能与重庆市EH人群的舒张压水平有关,GA4A4A基因型人群可能是EH患者血压升高的一个高危遗传因素.
To investigate the association between G8002A, +138A / - polymorphism and Essential hypertension (EH) in Endothelin-1 (ET-1) The genotypes of G8002A and + 138A / - polymorphisms in 95 EH patients and 150 normotensive subjects (NT) were detected by PCR-RFLP and PCR-SSP respectively. The results showed that + 138A / - The point genotypes were associated with diastolic pressure (p = 0.005), and the diastolic blood pressure of type 4A4A was 13 mmHg higher than that of type 3A3A (p = 0.005) and 12 mmHg higher than that of type 3A4A (p = 0.04) Point analysis showed that the diastolic blood pressure was significantly correlated with the average number of GA4A4A in EH patients as much as 20 mmHg (p <0.01), suggesting that A + insertion mutants of ET-1 gene + 138A / - And Chongqing EH population diastolic blood pressure level, GA4A4A genotype may be elevated blood pressure in EH patients with a high risk genetic factors.