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超氧化物歧化酶(EC1.15.1.1,简称SOD)广泛存在于需氧生物的各种组织中,它能催化超氧化物自由基(O_2~-)发生歧化反应: 由于SOD具有清除O_2~-自由基的能力,所以在防御氧的毒性、抗辐射损伤、抗炎症和抗衰老等方面具有重要作用,近年来对SOD的生物学意义和临床应用方面的研究颇引起生物学和医学界的重视。SOD的底物O_2~-是一种寿命很短的自由基,这造成了SOD活力测定上困难。至今除了应用脉冲辐射分解和电子自旋共振(ESR)技术可直接观察O_2~-在SOD催化下发生衰减的动力学变化过程外,一般都采用间接的活力测定方法,如细胞色素C还原法、四氮唑兰(NBT)法、光化学扩增
Superoxide dismutase (EC1.15.1.1, referred to as SOD) is widely present in various tissues of aerobic organisms and catalyzes the disproportionation reaction of superoxide radicals (O 2 -): Since SOD has the ability to scavenge O 2 - - the ability of free radicals, it plays an important role in the defense against oxygen toxicity, anti-radiation damage, anti-inflammatory and anti-aging and so on. In recent years, the research on the biological significance and clinical application of SOD has caused many problems in biology and medicine Pay attention. O_2 ~ -, a substrate of SOD, is a short-lived free radical, which makes it difficult to determine the activity of SOD. To date, indirect kinetic assays, such as cytochrome C reduction, are generally used, except for the use of pulsed radiation decomposition and electron spin resonance (ESR) techniques to directly observe the decay kinetics of O 2 - - catalyzed by SOD. Tetrazolium (NBT) method, photochemical amplification