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目的 观察茶多酚对 HL- 60细胞氧化损伤时 DNA双链残余率的影响。方法 采用 DNA荧光解旋法 (FADU)对 DNA双链残余率进行检测。结果 过氧化氢可以对细胞 DNA造成损伤 ,细胞浓度为 1 .5× 1 0 9/ L时 ,过氧化氢浓度在 0 .1～ 0 .5 mmol/ L时与DNA双链残余率有良好的线性关系。茶多酚浓度在 1 0～ 80 mg/ L时过氧化氢诱导的 DNA氧化损伤具有明显的抑制作用 (P<0 .0 5) ,但茶多酚浓度过高 ,DNA双链残余率有减少趋势。结论 过氧化氢可以造成 HL- 60细胞 DNA的氧化损伤 ,茶多酚具有保护细胞 ,抑制DNA氧化断裂程度 ,在一定浓度范围内 ,茶多酚的抗氧化作用与其浓度成正比 ,过高浓度的茶多酚则抗氧化能力下降。
Objective To investigate the effect of tea polyphenols on the residual DNA double-stranded DNA (DNA) residues in HL-60 cells during oxidative injury. Methods DNA double-stranded DNA residual rate was detected by DNA Fluorescence Unpinning (FADU). Results Hydrogen peroxide could damage cell DNA. When the cell concentration was 1.5 × 10 9 / L and the hydrogen peroxide concentration was between 0.1-1.5 mmol / L, the DNA double-stranded residue had a good Linear relationship. Hydrogen peroxide-induced DNA oxidative damage significantly inhibited the tea polyphenols concentration from 10 mg / L to 80 mg / L (P <0.05), but the tea polyphenol concentration was too high and the DNA double-strand residual rate was decreased trend. Conclusion Hydrogen peroxide can cause oxidative DNA damage in HL-60 cells. Tea polyphenols can protect cells and inhibit DNA oxidative damage. In a certain concentration range, the antioxidant activity of tea polyphenols is proportional to its concentration. Tea polyphenols decreased antioxidant capacity.