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目的：获取人雌激素受体（ＥＲ）及其抗体。方法：扩增编码ＥＲＢＣＤ区的基因，在大肠杆菌中表达ＭＳ２－ＥＲ融合蛋白。免疫动物，得抗ＥＲ血清，以此检测５０余例乳腺良、恶性肿瘤的ＥＲ表达，并与经典检测ＥＲ的ＤＣＣ法及进口抗ＥＲ单克隆抗体Ｃ－３１４结果相比较。结果：ＭＳ２－ＥＲ融合蛋白有天然ＥＲ。结构，其抗血清特异针对野生型ＥＲ，其ＩＨＣ结果与ＤＣＣ及Ｃ－３１４抗ＥＲ单克隆抗体有高符合率，既具备ＩＨＣ方法的特点，又免去需微波处理这一步骤。结论：自制抗ＥＲ血清可用来检测ＥＲ表达，为进一步获取其单克隆抗体及推广奠定基础。
Objective: To obtain human estrogen receptor (ER) and its antibody. Methods: The gene encoding ERBCD region was amplified and expressed in E. coli MS2-ER fusion protein. The animals were immunized with anti-ER serum to detect the ER expression of more than 50 cases of benign and malignant breast tumors. The results were compared with the results of classic DCC assay and imported anti-ER monoclonal antibody C-314. Results: The MS2-ER fusion protein has a native ER. Structure. The antiserum was specifically targeted to wild-type ER and its IHC result was in good agreement with DCC and C-314 anti-ER monoclonal antibodies. It not only possesses the characteristics of IHC but also eliminates the need of microwave treatment. Conclusion: Self-made anti-ER serum can be used to detect the expression of ER in order to further obtain its monoclonal antibody and promote the foundation.