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目的：研究三七总皂苷（ＰＮＳ）对神经细胞的保护作用。方法：采用原代培养的大鼠大脑皮层神经细胞，观察谷氨酸（５００μｍｏｌ·Ｌ－１，８ｍｉｎ），缺氧５ｈ再给氧不同时间（０，３，２４ｈ）对细胞的影响以及ＰＮＳ的作用。结果：ＰＮＳ２５，５０ｍｇ·Ｌ－１明显减少缺氧／再给氧损伤时细胞内酶的释放，减轻细胞形态学的改变，提高细胞的存活率。对谷氨酸介导的兴奋性毒性，ＰＮＳ也有一定的拮抗作用。结论：ＰＮＳ的脑保护作用机制可能与其抗兴奋性毒性和缺氧性损伤的作用有关。
Objective: To study the protective effect of Panax notoginseng saponins (PNS) on nerve cells. METHODS: Primary cultured rat cerebral cortical neurons were used to observe the effects of glutamate (500 μmol·L-1, 8 min), hypoxia for 5 h, and oxygen supply for different time (0, 3, 24 h) on the cells and PNS. effect. RESULTS: PNS25, 50 mg·L-1 significantly reduced the release of intracellular enzymes during hypoxia/reoxygenation injury, reduced the change of cell morphology and increased the cell survival rate. For glutamate-mediated excitotoxicity, PNS also has some antagonism. CONCLUSION: The protective mechanism of PNS in brain may be related to its anti-excitotoxic and anoxic effects.