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目的 研究 Vit E和硒对人髓系白血病细胞株 HL-60细胞凋亡和 c-myc基因表达的影响。方法 应用体外细胞培养法 ,DNA凝胶电泳技术、Northern印迹杂交技术。结果 Vit E( 10 0 μmol/L)和 Se( 8μmol/L)作用 2 4h后 ,HL-60细胞出现细胞凋亡现象。Vit E( 10 0 μmol/L)可以抑制 c-myc基因的表达 ,作用有显著性 ( P <0 .0 5 )。Se( 8μmol/L)无此作用。结论 抑制 c-myc基因的表达 ,诱导白血病细胞凋亡是 Vit E抑制白血病细胞增殖的重要途径之一。硒与 Vit E同样具有抗氧化作用 ,可诱导 HL-60细胞凋亡。但对 c-myc基因的表达却无影响 ,提示二者的作用机制是不同的
Objective To investigate the effects of Vit E and selenium on apoptosis and c-myc gene expression in human myeloid leukemia HL-60 cells. Methods In vitro cell culture, DNA gel electrophoresis, Northern blot hybridization. Results The apoptosis of HL-60 cells was observed after Vit E (10 μmol / L) and Se (8 μmol / L) for 24 hours. Vit E (100 μmol / L) could inhibit the expression of c-myc gene, with a significant effect (P <0.05). Se (8μmol / L) without this effect. Conclusion Inhibition of c-myc gene expression and induction of apoptosis in leukemia cells are one of the important ways Vit E inhibits the proliferation of leukemia cells. Selenium and Vit E also have antioxidant effects and induce apoptosis in HL-60 cells. But no effect on the expression of c-myc gene, suggesting that the mechanism of action of the two are different