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目的 :构建三种重构型人caspase - 8基因的原核表达载体 ,转染大肠杆菌并诱导其表达。方法 :将人caspase- 8催化结构域基因及两种大、小亚基基因次序颠倒的重构型人caspase - 8基因克隆入原核表达载体pBV2 2 0 ,转染大肠杆菌并诱导表达。结果 :成功构建了三种重构型人caspase- 8基因的原核表达载体。转染大肠杆菌后 ,经温度诱导 ,重构型人caspase - 8基因获得了高效的表达。结论 :在大肠杆菌中成功表达了三种重构型人caspase - 8基因。
OBJECTIVE: To construct prokaryotic expression vector of three reconstructed human caspase - 8 gene and transfect E. coli to induce its expression. Methods: The reconstructed human caspase - 8 gene with reverse cascade of caspase - 8 catalytic domain and two large and small subunit genes was cloned into prokaryotic expression vector pBV220 and transfected into E. coli for expression. Results: Three recombinant prokaryotic expression vectors of human caspase-8 gene were successfully constructed. After transfection with E. coli, the recombinant human caspase - 8 gene was highly expressed after temperature induction. Conclusion: Three recombinant human caspase - 8 genes were successfully expressed in E. coli.