论文部分内容阅读
目的采用气相色谱法测定沙棘籽油中甾醇含量,考察低甾醇沙棘籽油(sea buckthorn seed oil,SBSO)的制备工艺。方法采用皂化萃取法、络合法、脱胶碱炼法和吸附法分别制备低甾醇沙棘籽油,以注射用大豆油中甾醇含量为对照,通过气相色谱法对沙棘籽油中甾醇含量进行检测。结果硅胶吸附法制备的低甾醇沙棘籽油总甾醇含量显著降低,与沙棘籽毛油相比,占总甾醇90%左右的β-谷甾醇由19.56%降至6.31%。皂化萃取法、络合法、脱胶碱炼制备的低甾醇沙棘籽油甾醇含量均有不同程度的降低,β-谷甾醇含量分别为13.46%、8.22%和9.36%。结论硅胶吸附法精制的沙棘籽油总甾醇含量显著降低,该制备工艺操作相对简易,除杂效率高。
Objective To determine the content of sterols in seabuckthorn seed oil by gas chromatography and to study the preparation technology of sea buckthorn seed oil (SBSO). Methods Low sterol seabuckthorn seed oil was prepared by saponification, complexation, degumming and adsorption respectively. The content of sterol in seabuckthorn seed oil was determined by gas chromatography with the content of sterols in soybean oil for injection. Results The total sterol content of low sterol seabuckthorn seed oil prepared by silica gel adsorption was significantly lower than that of seabuckthorn seed oil, and the content of β-sitosterol, which accounted for about 90% of total sterols, decreased from 19.56% to 6.31%. Saponification, complexation, and degumming alkaline refining all decreased the content of oil sterols in sesto seabuckthorn seeds, and the contents of β-sitosterol were 13.46%, 8.22% and 9.36%, respectively. Conclusion The total sterol content of Seabuckthorn seed oil refined by silica gel adsorption method is significantly reduced. The preparation process is relatively simple and easy to remove impurities.