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目的:建立HPLC法同时测定气滞胃痛颗粒中白芍药苷、芍药苷、甘草苷、柚皮苷和新橙皮苷的含量。方法:采用依利特Hypersil C18色谱柱(4.6 mm×150 mm,5μm),以乙腈(A)-水(B)为流动相梯度洗脱(0～45 min,10%A→28%A),流速1.0mL.min-1,采用DAD检测器在200～400 nm波长处进行检测,柱温30℃。结果:调取不同波长的色谱图分别计算各成分的含量,白芍药苷(230 nm)、芍药苷(230 nm)、甘草苷(237 nm)、柚皮苷(283 nm)和新橙皮苷(283 nm)进样量分别在0.12～6.14μg(r=0.9998),0.40～7.92μg(r=0.9998),0.06～3.18μg(r=1.000),0.20～9.84μg(r=0.9999),0.12～6.14μg(r=0.9999)范围内呈现良好的线性关系。平均回收率(n=6)分别为98.1%,98.3%,98.9%,98.6%,97.1%;RSD分别为0.63%,0.69%,0.96%,1.5%,1.3%。结论:该方法快速简便,结果准确可靠,为气滞胃痛颗粒的全面质量控制提供科学的依据。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of paeoniflorin, paeoniflorin, liquiritin, naringin and neohesperidin in Qi-Zhi Wei Tong Granules. Methods: The elution was performed on a Hypersil C18 column (4.6 mm × 150 mm, 5 μm) with a gradient elution of acetonitrile (A) -water (B) (0-45 min, 10% A → 28% A) Flow rate 1.0mL.min-1, using DAD detector at 200 ~ 400 nm wavelength detection, the column temperature 30 ℃. Results: The chromatograms of different wavelengths were taken and the content of each component was calculated. The results showed that the contents of paeoniflorin (230 nm), paeoniflorin (230 nm), glycyrrhizin (237 nm), naringin (283 nm) (283 nm) were 0.12 ~ 6.14μg (r = 0.9998), 0.40 ~ 7.92μg (r = 0.9998), 0.06 ~ 3.18μg ~ 6.14μg (r = 0.9999) showed a good linear relationship. The average recoveries (n = 6) were 98.1%, 98.3%, 98.9%, 98.6% and 97.1%, respectively. The RSDs were 0.63%, 0.69%, 0.96%, 1.5% and 1.3%, respectively. Conclusion: The method is rapid and simple, the results are accurate and reliable, and provide a scientific basis for the total quality control of Qi stagnation stomach pain granules.