为研究特殊氨基酸在动物体内的生物感知和生理作用,测定了果蝇对20种氨基酸的饮食偏好性及进食量,并检测了不同氨基酸对果蝇运动水平的影响.研究发现,不同性别的果蝇对于不同种类的氨基酸具有独特的进食偏好,而苏氨酸可以显著影响果蝇的运动能力.进一步以苏氨酸为主要研究对象,对果蝇三龄幼虫给予饥饿、苏氨酸、蔗糖以及苏氨酸混合蔗糖的饮食处理,通过转录组高通量测序,分析组间基因差异.通过Gene Ontology和Kyoto Encyclopedia of Genes and Genomes富集分析发现,苏氨酸进食特异地对于果蝇的固醇类激素和氧化还原信号产生影响.进一步利用实时荧光定量PCR,对筛选获得的8个差异表达基因进行了验证,并通过对比成虫和幼虫发现不同发育阶段对于苏氨酸的响应可能存在较大差异.本工作为进一步深入研究特定氨基酸在动物中的感知和代谢调节提供了现象基础,并为发现其具体分子机制提供了基因表达的线索.“,”To investigate the perception and effects of specific amino acids on animals, we took Drosophila melanogaster as a model organism, measured the dietary preference and uptake of 20 amino acids as well as detected the effect of different amino acids on adult motility. We found that male and female Drosophila preferentially sense and consume different amino acids, and threonine specifically affects the motility of adult females. We then fed the third instar larvae with four dietary conditions ( starvation, threonine, sucrose, and threonine+sucrose) and analyzed the differentially expressed genes between groups by transcriptome profiling. Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that threonine affects steroid hormone and redox signaling. We further validated 8 genes by real-time fluorescence quantitative PCR in both larvae and adults,and found that the biological responses of threonine may depend on developmental stages. Our findings lay a foundation for additional in-depth investigation of the sensing and metabolic regulation of specific amino acids and provide clues of its molecular mechanism at the gene expression level.