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目的了解宁波市2016年流行性腮腺炎病毒(Mumps virus,Mu V)基因型特征。方法采用Vero/SLAM细胞分离Mu V,对Mu V毒株小疏水蛋白(Small hydrophobic protein,SH)基因进行逆转录-聚合酶链反应扩增和序列测定,与Gen Bank中参考株序列进行比较。结果宁波市2016年共分离到5株Mu V,均为F基因型,核苷酸及氨基酸之间同源性分别为95.07%和94.74%;与F基因型参考株Z77161同源性分别为96.42%-97.09%和94.74%-96.49%;与中国疫苗株S79同源性分别为80.66%-81.52%和73.68%-75.44%;与中国其他地区F基因型之间同源性分别为95.05%-97.42%和91.23%-96.49%。结论 2016年宁波地区Mu V流行株均为F基因型,未发现SH基因变异。
Objective To understand the characteristics of Mumps virus (M V Vs) genotypes in Ningbo City in 2016. Methods Mu V was isolated from Vero / SLAM cells. The small hydrophobic protein (SH) gene of Mu V was amplified by RT-PCR and compared with the reference strain in Gen Bank. Results Five Mu V strains were isolated from Ningbo City in 2016, all of which were F genotypes with 95.07% and 94.74% nucleotide and amino acid homology respectively. The homologies with the reference strain Z77161 of F genotype were 96.42 % -97.09% and 94.74% -96.49%, respectively. The homologies with Chinese vaccine strains S79 were 80.66% -81.52% and 73.68% -75.44%, respectively. The homologies with F genotypes in other regions of China were 95.05% 97.42% and 91.23% -96.49%. Conclusion In 2016, all the Mu V strains in Ningbo were F genotypes, and no SH gene mutation was found.