本实验采用一种单特异性,多克隆抗体,对刚分离的各类睾丸细胞,附睾精子和体外培养的支持细胞进行鸟氨酸脱羧酶免疫化学定位。实验结果表明:鸟氨酸脱羧酶样的物质,在睾丸的支持细胞、间质细胞、各类生殖细胞都存在,首次说明粗丝期精母细胞、圆精原细胞及后期精原细胞亦存在此物质。在附睾精子,鸟氨酸脱羧酶的抗原物质存在于其顶体。我们 In this study, a kind of monospecific, polyclonal antibody was used to perform immunochemical localization of ornithine decarboxylase to various testicular cells, epididymal sperm and supporting cells cultured in vitro. Experimental results show that: ornithine decarboxylase-like substances in the testicular support cells, interstitial cells, all kinds of germ cells are present, for the first time that sliver spermatocytes, round spermatogonia and post-spermatogonia also exist This material. In the epididymal sperm, ornithine decarboxylase antigen substances exist in its acrosome. we