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目的 :探讨形态学、免疫学、细胞遗传学和分子生物学方法 (MICM )分型对APL临床实用性及意义。方法 :对 2 1例急性早幼粒细胞白血病 (APL)进行了MICM的联合检测。结果 :显示形态学分型的确诊率为 90 .5 % (19/2 1) ;免疫学分析 :提示CD3 3 、CD13 、CD9、CD3 4 有一定阳性表达。尤其是CD3 3 、CD13 阳性表达高 ;染色体异常 :在初治2 0例APL中 ,t(15 ;17)检出率为 5 0 .0 % (10 / 2 0 ) ;采用RT/PCR技术检测 :AML RARα融合基因阳性率为 75 .0 %(15 / 2 0 )。结论 :形态学是诊断APL的基础 ,免疫学有辅助作用 ,t(15 ;17)及PML RARα融合基因阳性是APL生物学本质的反应 ,也是APL的诊断、ATRA治疗的选择、疗效评价及微小残留病监测的一个快速、准确且灵敏的方法
Objective: To investigate the clinical utility and significance of the morphology of morphological, immunological, cytogenetic and molecular biological methods (MICM) for APL. Methods: A total of 21 patients with acute promyelocytic leukemia (APL) were examined by combined detection of MICM. Results: The diagnostic accuracy of the morphological classification was 90.5 % (19/2 1). Immunological analysis indicated that CD3 3, CD13, CD9 and CD3 4 were positively expressed. In particular, the positive expression of CD3 3 and CD13 was high; chromosomal abnormalities: In the 20 cases of untreated APL, the detection rate of t(15;17) was 50% (10/20); RT/PCR was used to detect : The positive rate of AML RARα fusion gene was 75.0 % (15 / 20). Conclusion: Morphology is the basis for the diagnosis of APL, immunological aids, t(15;17) and PML RARα fusion gene positivity are responses to the biological nature of APL, but also APL diagnosis, ATRA treatment options, efficacy evaluation and micro A Fast, Accurate and Sensitive Approach to Surveillance of Residual Diseases