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将含有编码Ｖｉ抗原ＶｉａＢ基因片断的质粒转导进入宋内氏痢疾菌无毒株Ｓ７中，组建了重组菌株Ｓ７Ｖｉ。质粒电泳图谱显示重组菌株Ｓ７Ｖｉ中存在被转入的外源质粒带。重组株的生化特性没有改变。菌体凝集及Ｖｉ抗血清标记的ＳＰＡ菌液凝集反应证明在重组株的菌体表面，同时表达了Ｖｉ抗原和宋内氏毒菌的Ｏ抗原。以５×１０~８ＣＦＵ、１０×１０~８ＣＦＵ的重组株免疫近交系的ＬＩＢＰ小鼠，免疫小鼠对宋内氏毒株Ｓ６３攻击的保护率为６０％至９０％，对伤寒毒株Ｔｙ２攻击的保护率为２５％至４０％。
The plasmid containing the ViaB gene encoding the Vi antigen was transduced into the non-toxic strain S7 of S. to construct the recombinant strain S7Vi. Plasmid electrophoresis showed that there was exogenous plasmids transferred into the recombinant strain S7Vi. The biochemical characteristics of the recombinant strain did not change. The bacterial agglutination and Vi antiserum-labeled SPA bacterial agglutination showed that both the Vi antigen and the O antigen of Sonianensis were expressed on the surface of the recombinant strain. The inbred strains of LIBP mice were immunized with 5 × 10 ~ 8 CFU and 10 × 10 ~ 8 CFU of recombinant strains, and the protection rate of S501 challenge to the S63 strain was 60% -90% Attack protection rate of 25% to 40%.