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目的评价广西产何首乌中大黄素对人鼻咽癌裸鼠移植瘤的放射增敏作用。方法通过皮下注射肿瘤细胞的方法建立人鼻咽癌裸鼠移植瘤模型,荷瘤鼠随机分成7组:空白对照组、溶媒组、大黄素组、单纯放射治疗组、大黄素联合放射治疗组,其中大黄素组和联合治疗组按照药物高、低剂量各分为两个亚组。经处理后各组隔日测量移植瘤的最大径,计算肿瘤生长延缓天数,利用增敏系数评价大黄素的放射增敏作用,HE染色,观察瘤组织病理学特征,计算核分裂率。结果单纯照射组肿瘤生长受到一定程度的抑制,和对照组相比,肿瘤生长延缓时间为4.28 d;高、低剂量大黄素联合放疗组较其他实验组肿瘤生长明显受到抑制,和单纯照射组相比,肿瘤生长延缓时间分别为6.90和6.77 d(P<0.05)。高、低剂量大黄素对鼻咽癌裸鼠移植瘤放射治疗的增敏值分别为1.612和1.601。高、低两个剂量大黄素联合放射治疗组的核分裂率分别是(3.80±0.45)%和(3.43±0.34)%,与对照组比较差异有统计学意义(P<0.05)。结论大黄素对人鼻咽癌裸鼠移植瘤具有放射增敏作用。
Objective To evaluate the radiosensitization effect of emodin from Guangxi Polygonum multiflorum on human nasopharyngeal carcinoma xenografts in nude mice. Methods Human nasopharyngeal carcinoma xenograft model was established by subcutaneous injection of tumor cells. The tumor-bearing mice were randomly divided into 7 groups: blank control group, vehicle group, emodin group, radiotherapy alone group, emodin combined with radiotherapy group, The emodin group and the combination therapy group were divided into two subgroups according to the drug high and low dose. After treatment, the maximum diameter of the transplanted tumor was measured every other day. The days of tumor growth retardation were calculated. The sensitization coefficient was used to evaluate the radiosensitization of emodin. HE staining was used to observe the histopathological features of the tumor and calculate the mitotic rate. Results Compared with the control group, the tumor growth retardation was 4.28 days in pure irradiation group. The tumor growth was significantly inhibited in high and low dose emodin combined with radiotherapy group compared with the other experimental groups, The tumor growth delay time was 6.90 and 6.77 days (P <0.05), respectively. The sensitivities of high and low dose emodin to radiotherapy of nasopharyngeal carcinoma in nude mice were 1.612 and 1.601 respectively. The rates of mitosis in high dose and low dose emodin combined with radiotherapy group were (3.80 ± 0.45)% and (3.43 ± 0.34)%, respectively, which were significantly different from those in control group (P <0.05). Conclusion Emodin can radiosensitize human nasopharyngeal carcinoma xenografts in nude mice.