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目的探讨深圳市宝安区乙型肝炎患者血清中乙肝病毒(HBV)基因型的分布情况,了解各基因型与HBV-DNA水平、HBeAg、肝损程度、抗病毒疗效、疾病进展和预后关系。方法采用S基因片段测序的基因分型法,通过上下游引物经PCR反应体系和操作程序扩增S基因片段,经1.5%琼脂糖凝胶电泳,用3730双向测序技术,将130例HBV-DNA阳性患者测序结果在NCBI上进行HBV基因分型,得出基因型。结果宝安区HBV基因型只有B型和C型,无其他型和混合型,B型占55.74%,C型占44.26%,无性别和年龄分布差异(P>0.05)。B型与C型HBV-DNA水平无显著性差异(P>0.05),B基因型HBeAg阳性率较C基因型高,C型肝损程度比B型重,进展为肝硬化、肝细胞性肝癌的几率高,两者相比有显著性差异(P<0.01)。结论研究病例HBV基因型以B型为主,C型次之。C基因型患者病情重,进展快,预后差,值得引起临床医生重视。
Objective To investigate the distribution of hepatitis B virus (HBV) genotypes in the serum of patients with hepatitis B in Bao’an District of Shenzhen City and to understand the relationship between genotypes and HBV-DNA levels, HBeAg, liver damage, anti-viral efficacy, disease progression and prognosis. Methods S gene fragment was sequenced by upstream and downstream primers. The S gene fragment was amplified by PCR reaction system and operating procedure. The 1.5% agarose gel electrophoresis was used to screen 130 S-DNA fragments by 3730 two-dimensional sequencing. Positive Patient Sequencing Results HBV genotyping on NCBI resulted in genotypes. Results The genotypes of HBV in Baoan District were only B and C, no other type and mixed type. The B genotype accounted for 55.74% and the C genotype accounted for 44.26%. There was no significant difference in gender and age distribution (P> 0.05). There was no significant difference in HBV-DNA between B and C (P> 0.05). The positive rate of HBeAg in B genotype was higher than that in C genotype, and the degree of C-type liver damage was heavier than that of B genotype. The progression was cirrhosis, hepatocellular carcinoma (P <0.01). There was a significant difference between the two groups (P <0.01). Conclusions The genotypes of HBV in the study cases are mainly B type and C type. C genotype patients with severe illness, rapid progress, poor prognosis, it is worth the attention of clinicians.