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目的探讨环氧化酶-2(cyclooxygenase-2,COX-2)基因在非小细胞肺癌(NSCLC)中的表达及其与血管内皮生长因子(VEGF)和CD105的关系。方法逆转录聚合酶链反应(RT-PCR)方法检测64例NSCLC组织及相对应的正常组织中COX-2mRNA的表达,免疫组织化学法检测VEGF、CD105蛋白的表达,以CD105计数微血管密度(MVD)。结果NSCLC组织COX-2mRNA表达阳性率为67.2%,相应正常组织则为14.1%(P<0.01);NSCLC组织COX-2mRNA表达强度高于相应正常组织(P<0.01)。COX-2mR-NA的表达与VEGF的表达显著相关(P<0.01),COX-2阳性癌组织的MVD高于COX-2阴性表达者(P<0.05)。结论NSCLC组织中COX-2表达上调且微血管被激活,COX-2可能通过诱导VEGF表达或与VEGF、CD105协同作用而促进肿瘤血管形成。
Objective To investigate the expression of cyclooxygenase-2 (COX-2) gene in non-small cell lung cancer (NSCLC) and its relationship with vascular endothelial growth factor (VEGF) and CD105. Methods The expression of COX-2 mRNA in 64 NSCLC tissues and corresponding normal tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR). The expressions of VEGF and CD105 were detected by immunohistochemistry. ). Results The positive rate of COX-2 mRNA expression in NSCLC was 67.2%, while the corresponding normal tissue was 14.1% (P <0.01). The expression of COX-2 mRNA in NSCLC tissues was higher than that in corresponding normal tissues (P <0.01). The expression of COX-2mR-NA was significantly correlated with the expression of VEGF (P <0.01). The MVD of COX-2 positive cancer tissue was higher than that of COX-2 negative expression (P <0.05). Conclusion COX-2 is upregulated and microvessel is activated in NSCLC. COX-2 may promote tumor angiogenesis by inducing VEGF expression or synergistically with VEGF and CD105.