本研究应用甲基化酶温育法检测２１例胃癌手术标本中各部位粘膜细胞总基因组ＤＮＡ甲基化水平，发现癌区明显低于癌旁和正常粘膜组织区，而后两者无显著差异。ＤＮＡ甲基化水平下降之幅度与肿瘤细胞侵及深度和组织学类型无关，而与肿瘤的大体形态、胃周淋巴结受侵情况及肿瘤细胞的分化程度有关。 In this study, the methylation level of total genomic DNA in mucosal cells was detected in 21 surgical specimens of gastric cancer by methylation enzyme incubation method. It was found that the cancer area was significantly lower than that in the paracancerous and normal mucosal areas. There was no significant difference between the latter two groups. The extent of DNA methylation decline was not related to tumor invasion depth and histological type, but related to the general morphology of the tumor, the involvement of gastric lymph nodes, and the degree of differentiation of tumor cells.