人类分娩开始的早期表现之一是子宫内前列腺素合成增加,但其机制尚不清楚。本文通过Northern印迹分析,探讨了分娩前后胎膜和胎盘中磷脂酶A_2、脂皮素(Annexins)Ⅰ、Ⅱ、Ⅴ基因表达的改变,并讨论其与前列腺素合成的关系。 分娩前、后的胎膜和胎盘分别来自10例临产、足月、无妊娠合并症的剖宫产患者以及10例足月、无妊娠合并症的自发分娩者。取得的标本用磷酸缓冲盐 One of the early manifestations of human delivery begins with increased prostaglandin synthesis in the womb, but the mechanism is not clear. In this study, the changes of phospholipase A_2, Annexin Ⅰ, Ⅱ and Ⅴ gene expressions in fetal membranes and placenta before and after childbirth were investigated by Northern blot analysis and their relationship with prostaglandin synthesis was discussed. Prenatal and postnatal membranes and placenta were from 10 cesarean section patients with labor, full-term, non-pregnancy complications, and 10 cases of spontaneous delivery of full-term, non-pregnancy complications. The specimens obtained were phosphate buffered saline