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目的探讨新疆准噶尔盆地鼠疫野外分离株的营养需求。方法应用定性和定量两种方法相结合分析准噶尔盆地28株鼠疫菌对11种氨基酸的营养需求。结果准噶尔盆地28株鼠疫菌株中,低营养型1株(No.25,Phe+)占检测菌株的3.57%;色氨酸依赖1株(No.24,Try-)占检测菌株的3.57%;苯丙氨酸、甲硫氨酸、缬氨酸依赖,异亮氨酸半依赖1株(No.21,Phe-、Met-、Val-、Ile±)占检测菌株的3.57%;余下25株表现为对苯丙氨酸、甲硫氨酸、缬氨酸依赖(Phe-、Met-、Val-)占检测菌株的89.3%。结论新疆准噶尔盆地鼠疫菌株的营养型主要表现为对苯丙氨酸、甲硫氨酸、缬氨酸依赖(Phe-、Met-、Val-)。
Objective To investigate the nutritional requirements of the outbreaks of plague in Xinjiang Junggar Basin. Methods The qualitative and quantitative methods were used to analyze the nutritional requirements of 11 Yersinia pestis isolates from 28 strains of Yersinia pestis in the Junggar Basin. Results Among the 28 plague isolates in Junggar Basin, 1 strain (No.25, Phe +) accounted for 3.57% of the tested strains, 1 strain (No.24, Try-) accounted for 3.57% of the tested strains, benzene 1 (No.21, Phe-, Met-, Val-, Ile ±) of alanine, methionine, valine and isoleucine accounted for 3.57% of the tested strains; the remaining 25 For p-phenylalanine, methionine, valine dependence (Phe-, Met-, Val-) accounted for 89.3% of the test strains. Conclusion The nutritional types of the plague strains in Junggar Basin in Xinjiang are mainly phenylalanine, methionine and valine (Phe-, Met-, Val-).