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目的探讨川芎嗪(LGT)、异丙酚(PRO)联合使用对肝缺血-再灌注损伤(HIRI)时肝细胞能量代谢的影响及其机制。方法实验兔40只,随机分为肝缺血-再灌注组(A组)和肝缺血-再灌注+LGT治疗组(B组)、肝缺血-再灌注+PRO治疗组(C组)和肝缺血-再灌注+LGT+PRO治疗组(D组)。在再灌注45min时,分别检测肝组织内三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)含量及总腺苷酸量(TAN)、能荷(EC)、丙二醛浓度(MDA)、超氧化物歧化酶活性(SOD)、一氧化氮代谢产物(NO2-/NO3-)水平。结果与A组比较,B、C、D组肝组织内ATP、EC、NO2-/NO3-及SOD活性均明显增高(P<0.05和P<0.01),MDA含量显著减少(P<0.01)。结论LGT联用PRO可通过降低体内氧自由基水平、提高一氧化氮(NO)水平,而改善缺血-再灌注损伤肝脏的能量代谢。
Objective To investigate the effect of ligustrazine (LGT) and propofol (PRO) on the energy metabolism of hepatocytes during hepatic ischemia-reperfusion injury (HIRI) and its mechanism. Methods Forty rabbits were randomly divided into two groups: ischemia-reperfusion (A) group and ischemia-reperfusion + LGT group (B) And liver ischemia-reperfusion + LGT + PRO treatment group (group D). At 45 min after reperfusion, the levels of ATP, ADP, AMP and TAN, EC, Aldehyde concentration (MDA), superoxide dismutase activity (SOD) and nitric oxide metabolites (NO2- / NO3-). Results Compared with group A, the activity of ATP, EC, NO2- / NO3- and SOD in group B, C and D were significantly increased (P <0.05 and P <0.01), and the content of MDA was significantly decreased (P <0.01). Conclusions LGT combined with PRO can improve the energy metabolism of liver in rats with ischemia-reperfusion injury by decreasing the level of oxygen free radicals in the body and increasing the level of nitric oxide (NO).