通过在由(S)-(N)-(2-萘基)丙氨酸衍生而成的“刷型”手性固定相上采用离子对色谱分离氨基酸的3,5-二硝基苯甲酰衍生物,探讨了非手性离子对试剂的浓度对溶质保留值和选择性的影响。使用甲醇与磷酸盐缓冲溶液组成的溶剂为流动相,考察了甲醇含量对手性分离的影响。另外,通过在不同构型的手性固定相上做对照实验,证明在结构相同、构型相反的手性固定相上,对映体的出峰顺序是相反的。 The 3,5-dinitrobenzoyl amino acid was isolated by ion-pair chromatography on a “brush” chiral stationary phase derivatized with (S) - (N) - Derivatives, the influence of the concentrations of achiral ion pair reagents on solute retention and selectivity was discussed. The solvent of methanol and phosphate buffer solution was used as the mobile phase, and the effect of methanol content on chiral separation was investigated. In addition, the control experiments on chiral stationary phases of different configurations prove that the order of the enantiomers on the chiral stationary phase of the same structure and the opposite configuration is opposite.