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目的:观察丹芪合剂对糖尿病大鼠肾组织蛋白激酶B(PKB/Akt1)表达的影响,探讨其保护肾脏的机制。方法:SD大鼠随机分为正常对照组、糖尿病模型组、糖尿病丹芪合剂组(2 g.kg-1)和依那普利组(10 mg.kg-1)。单次尾静脉注射链脲佐菌素复制糖尿病模型。生化方法检测血糖、尿蛋白和血肌酐。免疫组化检测肾组织Akt1、E-钙黏素(E-cadherin)、纤维连接蛋白(FN)蛋白表达;Western blot检测α-平滑肌肌动蛋白(α-SMA)蛋白水平;RT-PCR检测Akt1 mRNA水平。结果:丹芪合剂明显降低DM大鼠肾组织Akt1蛋白和mRNA表达(P<0.01);同时上调E-cadherin蛋白表达并下调α-SMA表达,使FN沉积减少(P<0.01);降低DM大鼠血肌酐和尿蛋白水平(P<0.05)。丹芪合剂组与依那普利组之间无统计学差异。结论:丹芪合剂可能通过抑制Akt信号分子,进而抑制上皮细胞-间充质细胞转化,减少FN沉积,对糖尿病大鼠肾脏起保护作用。
Objective: To observe the effects of Danqi Mixture on the expression of protein kinase B (PKB / Akt1) in kidney of diabetic rats and its mechanism of protecting kidneys. Methods: SD rats were randomly divided into normal control group, diabetic model group, diabetic Danqi mixture group (2 g.kg-1) and enalapril group (10 mg.kg-1). Single tail vein injection of streptozotocin in the replication of diabetic model. Biochemical detection of blood glucose, urine protein and serum creatinine. The expression of Akt1, E-cadherin and FN in renal tissue were detected by immunohistochemistry. The protein level of α-SMA was detected by Western blot. The expressions of Akt1, mRNA level. Results: Danqi mixture significantly reduced the expression of Akt1 protein and mRNA in DM rats (P <0.01), up-regulated the expression of E-cadherin protein and down-regulated the expression of α-SMA, and decreased the deposition of FN (P <0.01) Serum creatinine and urinary protein levels (P <0.05). Danqi mixture group and enalapril group no significant difference. Conclusion: Danqi mixture may protect the kidneys of diabetic rats by inhibiting Akt signaling molecules, thereby inhibiting the transformation of epithelial cells - mesenchymal cells and reducing FN deposition.