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Soybean is one of the most important sources of edible oil and proteins in the world.However,it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production.The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation.In this study,the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique.The lines were the T,of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene,and were positive by Southern Blot analysis.The resistance difference was studied through comparing the death percentage of transgenic soybean with the control.The results showed that four lines were more resistant to P.sojae,whereas other one had no significant difference in comparison with the control.These transgenic soybean lines with enhanced resistance to P.sojae will be useful in soybean resistance breeding.
Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation.In this study, the resistance to five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T, of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was been through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P. sojae, the other one had no significant difference in comparison with the control.These transgenic soybean lines with enhanced resistance to P.sojae will be useful in soybean resi stance breeding.