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目的探讨黄芪糖蛋白(HQGP)对大鼠佐剂性关节炎(adjuvant arthritis,AA)的治疗作用。方法将雄性Wistar大鼠随机分为正常组、模型组、雷公藤甲素组及HQGP低、中、高剂量组。于右后足跖皮内注射弗氏完全佐剂(CFA)建立大鼠AA模型,1周后给予相应的药物干预;2周后观察致炎侧踝关节肿胀程度以及膝关节滑膜组织形态学的影响,并检测各组大鼠外周血血清细胞因子γ-干扰素(IFN-γ)的水平。结果在治疗两周后,雷公藤甲素组与HQGP中、高剂量组大鼠致炎侧踝关节肿胀以及膝关节滑膜组织增生与模型组相比均有明显改善。HQGP高剂量组大鼠外周血血清IFN-γ水平显著升高。结论HQGP在腹腔注射剂量为3.5mg/kg和7.0mg/kg时,均对AA大鼠的关节炎症有显著改善作用;高剂量HQGP对AA大鼠外周血血清IFN-γ水平有升高作用,这说明在改善AA关节炎症的同时,HQGP还可能提高AA大鼠对抗其他感染的能力。
Objective To investigate the therapeutic effect of astragalus polysaccharide (HQGP) on adjuvant arthritis (AA) in rats. Methods Male Wistar rats were randomly divided into normal group, model group, triptolide group and HQGP low, medium and high dose groups. AA model was established by injecting Freund’s complete adjuvant (CFA) intradermally into the right hind paw. One week later, corresponding drug intervention was given. After 2 weeks, the extent of swelling of ankle joint and synovial tissue morphology The levels of serum IFN-γ in peripheral blood of rats in each group were measured. Results After treatment for two weeks, the swelling of the ankle joint on the inflamed side and the proliferation of synovial tissue in the knee joint of triptolide and HQGP middle and high dose groups were significantly improved compared with the model group. HQGP high-dose group of peripheral blood serum IFN-γ levels were significantly increased. Conclusions HQGP can significantly improve the joint inflammation of AA rats when intraperitoneal injection of 3.5mg / kg and 7.0mg / kg intraperitoneal injection of high dose of HQGP can increase the level of serum IFN-γ in AA rats, This suggests that while improving AA joint inflammation, HQGP may also improve AA rats’ ability to fight other infections.