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目的观察参青粉(参三七、青黛)对三硝基苯磺酸(TNBs)诱导的溃疡性结肠炎大鼠结肠黏膜低氧诱导因子-1(HIF-1)、血管内皮生长因子(VEGF)的影响,探讨其作用机制。方法将60只SD大鼠,分为正常组、模型组、SASP组和参青高、中、低剂量组,每组10只。除正常组外,其余各组采用三硝基苯磺酸(TNBs)诱导溃疡性结肠炎模型。造模后第3天开始各组给予相应的干预措施,连续干预7天后处死大鼠。采用免疫组化及Real time-PCR的方法测定各组大鼠结肠黏膜HIF-1及VEGF含量。结果与正常组比较,模型组大鼠结肠黏膜HIF-1和VEGF免疫组化指数、相对mRNA表达升高,差异有统计学意义(P<0.01)。与模型组比较,参青高、中、低剂量组大鼠结肠黏膜HIF-1和VEGF免疫组化指数、相对mRNA表达均下降,差异有统计学意义(P<0.01)。参青各剂量组间比较,高剂量组HIF-1免疫组化指数较中低剂量组升高,差异有统计学意义(P<0.05),而高、中剂量组VEGF免疫组化指数与低剂量组差异有统计学意义(P<0.05);参青高、中剂量组HIF-1和VEGF相对mRNA表达,与低剂量组比较,差异有统计学意义(P<0.05)。结论参青粉能有效下调溃疡性结肠炎HIF-1及VEGF的表达,改善肠道缺氧环境,修复肠道屏障,达到缓解大鼠溃疡性结肠炎的效应。
Objective To observe the effect of ginseng powder (ginseng and ginseng) on the expression of hypoxia inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) in colonic mucosa of rats with ulcerative colitis induced by trinitrobenzene sulfonic acid ), To explore its mechanism of action. Methods Sixty Sprague-Dawley rats were divided into normal group, model group, SASP group and high-dose, middle-dose and low-dose group. In addition to the normal group, the other groups using trinitrobenzene sulfonic acid (TNBs) induced ulcerative colitis model. On the 3rd day after model establishment, corresponding interventions were given to each group, and rats were sacrificed after 7 days of continuous intervention. The contents of HIF-1 and VEGF in colonic mucosa of rats in each group were determined by immunohistochemistry and Real time-PCR. Results Compared with normal group, the expression of HIF-1 and VEGF in colonic mucosa of model group increased, the relative mRNA expression increased, the difference was statistically significant (P <0.01). Compared with model group, the expression of HIF-1 and VEGF in colonic mucosa of high, medium and low dose group decreased significantly (P <0.01). Compared with the middle-low dose group, the HIF-1 immunohistochemical index of high dose group increased significantly (P <0.05), while the expression of VEGF in high and middle dose group was lower (P <0.05). Compared with low dose group, the expression of HIF-1 and VEGF mRNA in high and middle dose group was significantly lower than that in low dose group (P <0.05). Conclusion SSP can effectively reduce the expression of HIF-1 and VEGF in ulcerative colitis, improve the intestinal hypoxia environment, repair the intestinal barrier, and relieve the ulcerative colitis in rats.