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目的:观察自发性高血压大鼠(SHR)血管外周脂肪组织释放血管舒张因子功能的改变及他汀类药物干预的影响。方法:SHR10周龄后,分别给予阿托伐他汀钙50mg/kg.d,血脂康2400mg/kg.d干预16周。观察阿托伐他汀钙干预组(SHR-A)、血脂康干预组(SHR-X)、对照SHR组和WKY组的血压(SBP)变化;于26周龄,把各组大鼠的相邻的两段胸主动脉环分为血管外周脂肪亚组和裸血管亚组,予10-6mmol/L苯肾上腺素(PHE)刺激,比较两亚组血管收缩力的差异;用液体转移的方法,观察孵育血管外周脂肪组织的培养液对裸血管张力的影响。结果:①WKY组、SHR-A组、SHR-X组SBP实验前后无显著变化,SHR组的SBP实验结束时显著高于实验开始时;②WKY组、SHR-A组、SHR-X组血管外脂肪亚组的收缩力低于裸血管亚组的收缩力,而SHR两血管亚组的收缩力无差别;③把WKY组,SHR-A组,SHR-X组孵育的血管外脂肪的培养液转移到裸血管均诱发其快速舒张,而SHR组则无显著血管舒张反应。结论:WKY的血管外周脂肪组织释放一种可转移性血管舒张因子,降低血管对苯肾上腺素的反应性,调节血管功能。而SHR的血管外周脂肪组织这种血管调节作用减弱;血管外周脂肪组织这种功能异常可能是高血压血管功能异常的病理基础之一。他汀类药物治疗在修复SHR血管外周脂肪组织这种功能异常的同时,还能减缓SHR血压上升。
Objective: To observe the function of vascular relaxing factor released from peripheral vascular tissue of spontaneously hypertensive rats (SHR) and the effects of statins intervention. Methods: SHR 10 weeks of age, were given atorvastatin calcium 50mg / kg.d, Xuezhikang 2400mg / kg.d intervention for 16 weeks. The changes of SBP in atorvastatin calcium intervention group (SHR-A), Xuezhikang intervention group (SHR-X), control SHR group and WKY group were observed. At 26 weeks old, Of the thoracic aortic rings were divided into vascular subcutaneous fat subgroup and bare vascular subgroup, to 10-6mmol / L phenylephrine (PHE) stimulation, comparing two subgroups of vascular contractility differences; using liquid transfer method, Observe the effects of culture medium of vascular adventitial adipose tissue on bare vascular tension. Results: ① There was no significant change before and after SBP in WKY group, SHR-A group and SHR-X group, SBP in SHR group was significantly higher than the beginning of SBP experiment. ②WKY, SHR-A and SHR- The contractility of the subgroups was lower than that of the bare subgroups, while there was no difference in the contractility of the two subgroups of SHRs. ③ The extravascular fat culture incubated with WKY, SHR-A and SHR-X groups was transferred To the naked blood vessels were induced rapid diastolic, while no significant SHR group vasodilation. Conclusion: WKY can release a kind of vasodilatory factor in the peripheral blood adipose tissue, reduce the reactivity of blood vessel to phenylephrine and regulate the function of blood vessel. However, the vascular regulation of peripheral vascular adipose tissue of SHR is weakened. This dysfunction of vascular peripheral adipose tissue may be one of the pathological basis of vascular dysfunction of hypertension. Statin therapy can repair SHR vascular dysfunction of peripheral fat at the same time, but also can reduce SHR blood pressure.