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目的:探讨葡萄糖调节蛋白58(glucose-regulated protein 58,GRp58)GRp58在非小细胞肺癌组织中的表达情况及其与肺癌临床病理特征间的关系。方法:采用蛋白印迹法及免疫组化法检测40例非小细胞肺癌组织及配对癌旁组织中GRp58的表达及定位情况,并分析GRp58表达量与肺癌临床病理特征间的关系。结果:GRp58主要存在于非小细胞肺癌的细胞胞质中,非小细胞肺癌组织中GRp58的表达量(0.635±0.361)低于配对癌旁组织(0.899±0.245),差异有统计学意义(P<0.05),且GRp58的表达量与非小细胞肺癌临床分期有关(P<0.05),而与患者的年龄、性别、病理类型、组织学分化程度、淋巴结转移及吸烟情况均无关(P>0.05)。结论:GRp58表达量与非小细胞肺癌的临床分期有关,其低表达与非小细胞肺癌晚期可能相关。
Objective: To investigate the expression of GRp58 in non-small cell lung cancer (GRP58) and its relationship with the clinicopathological features of lung cancer. Methods: The expression and localization of GRp58 in 40 non-small cell lung cancer tissues and matched paracancerous tissues were detected by Western blotting and immunohistochemistry. The relationship between the expression of GRp58 and the clinicopathological features of lung cancer was analyzed. Results: GRp58 mainly existed in the cytoplasm of non-small cell lung cancer. The expression of GRp58 in non-small cell lung cancer (0.635 ± 0.361) was lower than that in paracancerous tissues (0.899 ± 0.245), with significant difference (P <0.05). The expression of GRp58 was correlated with the clinical stage of non-small cell lung cancer (P <0.05), but not with the age, gender, pathological type, histological differentiation, lymph node metastasis and smoking ). Conclusion: The expression level of GRp58 is correlated with the clinical stage of non-small cell lung cancer. The low expression of GRp58 may be related to the late stage of non-small cell lung cancer.