论文部分内容阅读
肺癌是全世界癌症死亡中的一个主要的原因。除吸烟外,一些肺癌患者的发病与氡气污染相关。该研究采用包括染色体分选、正向和反向染色体涂色技术,分析了两株肺腺癌细胞系A549和GLC-82的核型特征。A549和GLC-82细胞系都属于非小细胞肺癌细胞系,但诱因不同,后者来源于一个长期生活在氡气污染环境肺癌病人的癌组织。染色体涂色结果表明,这两株肺癌细胞系发生了复杂的染色体重排。在A549和GLC-82细胞系中,除正常染色体拷贝数变化外,还分别存在13条和24条畸变染色体。约一半的畸变染色体是通过非相互易位形成的,其余的畸变染色体则是通过一些正常染色体的片段缺失或重复而产生的。尽管这两株肺癌细胞系都没有共同的畸变染色体,但它们似共享两个染色体易位断裂点:HSA8q24和12q14。
Lung cancer is a leading cause of cancer deaths worldwide. In addition to smoking, some lung cancer patients are associated with radon contamination. In this study, the karyotype characteristics of two lung adenocarcinoma cell lines, A549 and GLC-82, were analyzed using chromosomal sorting, forward and reverse chromosome smear techniques. Both A549 and GLC-82 cell lines belong to the non-small cell lung cancer cell line, but with different causes, the latter derived from a long-term cancer patient living with lung cancer in a radon-polluted environment. Chromosomal staining showed that the two lung cancer cell lines undergo complex chromosomal rearrangements. In A549 and GLC-82 cell lines, there are 13 and 24 aberrant chromosomes, respectively, in addition to the normal copy number changes. About half of the aberrant chromosomes are formed by non-reciprocal translocations and the rest of the aberrant chromosomes are produced by deletion or duplication of fragments of some normal chromosomes. Although both lung cancer cell lines do not share a common aberrant chromosome, they share two chromosomal translocation breakpoints: HSA8q24 and 12q14.