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1989年10月沪县发生腹泻流行,病人畏寒、低热、腹泻、腹痛、里急后重,便频每日数次,粘液便、水样便或血性便,拟诊为细菌性痢疾。采样正在服药和新发病人肛拭子22个、混合水样1个,按肠道致病菌常规分离获EIECO_(152)K一株,现报告于后。1 材料和方法经G.N6～8h增菌后转种S.S平皿,选择培养,挑取可疑菌落于克氏双糖纯培分离到??福氏2a型12株、福氏1型1株;另一株具痢疾杆菌特征,但又不完全相同,据近年来腹泻病原菌研究进展资料报道疑为EIEC,逐做以下试验:(1)初步生化试验(赖氨酸脱羧酶、动力);
In October 1989, a prevalence of diarrhea occurred in Shanghai County. The patients were chills, fever, diarrhea, abdominal pain, tenesmus, frequent multiple times a day, mucus, water samples or bloody stools. The patient was diagnosed with bacillary dysentery. Twenty-two samples of anal swabs were taken and newly diagnosed. One sample was mixed with water sample, and one strain of EIECO_ (152) K was routinely isolated according to pathogenic bacteria of the intestine. MATERIALS AND METHODS Transgenic SS plates were obtained after enrichment by G.N6-8h. The suspicious colonies were picked and cultured in Klebsiella pneumoniae pure culture to isolate 12 strains of Staphylococcus aureus 2a and 1 strain of Staphylococcus aureus. Another strain with the characteristics of Shigella, but not exactly the same, according to recent progress in the research report on pathogens of diarrhea suspect EIEC, by the following tests: (1) preliminary biochemical tests (lysine decarboxylase, power);