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目的在基因转录水平了解肝再生中肝细胞的生长和分化情况。方法用搜集网站资料和查阅相关论文等方法获得参与肝细胞生长和分化基因,用大鼠基因组230 2.0芯片检测它们在大鼠肝再生(LR)中的表达情况,通过比较手术组和假手术组中基因表达差异性以确定上述基因中的肝再生相关基因。结果初步证实上述基因中110个基因与肝再生相关。肝再生启动(PH后0.5~4h)、G0/G1过渡(PH后4~6h)、细胞增殖(PH后6~66h)、细胞分化和组织结构功能重建(PH后72~168h)等4个阶段起始表达的基因数为63、11、43和3,基因总表达的次数为63、43、101和80,表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们共表达上调488次,下调248次,分为6种表达方式,表明肝再生中细胞生理生化活动的多样性和复杂性。结论肝细胞生长和分化贯穿于整个肝再生中。
Objective To understand the growth and differentiation of hepatocytes during liver regeneration at the gene transcription level. Methods The genes involved in the growth and differentiation of hepatocytes were obtained by collecting the website data and consulting related papers. The rat genomic 230 2.0 was used to detect the expression of the genes in rat liver regeneration. By comparing the two groups, Differences in gene expression to determine genes associated with liver regeneration in these genes. The results initially confirmed that 110 genes in the above genes were associated with liver regeneration. 4 (6 hours after PH), 4 ~ 6 hours after PH (6 ~ 66 hours after PH), 4 cycles of cell differentiation and functional reconstruction (72 ~ 168 hours after PH) The number of genes expressed at the beginning of the stage was 63, 11, 43 and 3, and the total number of the genes was 63, 43, 101 and 80, indicating that the related genes are mainly expressed at the initiation of liver regeneration and play roles in different stages. They were upregulated 488 times, down 248 times, divided into six kinds of expression, indicating the diversity and complexity of physiological and biochemical activities of cells in liver regeneration. Conclusion Hepatocyte growth and differentiation throughout the entire liver regeneration.