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目的评价MR测定大鼠脑部血氧饱和度的可重复性,探讨MR的低血氧饱和度与组织缺氧和胶质肉瘤模型内的缺氧与坏死之间的关系,评估MR血氧饱和度参数对于显示9L胶质肉瘤抗血管生成(索拉菲尼)治疗后改变的能力。材料与方法本实验获得法国农业部的许可。48只雄性大鼠接受9L胶质肉瘤的种植并随机分为(索拉菲尼)治疗组与未治疗组。在治疗前1d与开始治疗后1、3、5和8d,采集MR血容量分数与计算血氧饱和度参数。评价活体MR血氧饱和度测量与离体哌莫硝唑缺氧染色评估之间的相关性。统计学分析包括配对与独立样本t检验以及Pearson参数检验。结果健康组织的MR血氧饱和度测量结果与文献中的数值相符,并且具有可重复性[所有动物平均为(68.0±6.5)%]。在未治疗肿瘤组的缺氧坏死区,MR血氧饱和度测量与免疫组织化学分析具有相关性(R2=0.81)。在抗血管生成治疗肿瘤组,肿瘤的MR血氧饱和度测量与健康组织相比有所减低(P<0.001)。结论研究结果显示MR血氧饱和度测量具有可重复性,可作为脑肿瘤缺氧的活体检测方法和抗血管生成治疗后缺氧的敏感指标。
Objective To evaluate the reproducibility of MR measurements of brain oxygen saturation in rats and to explore the relationship between hypoxemia of MR and hypoxia and necrosis in hypoxic and glioma sarcoma models and to evaluate MR oxygen saturation The degree parameter is indicative of the ability of 9L gliosarcoma to change after anti-angiogenic (sorafenib) treatment. Materials and Methods This experiment was licensed by the French Ministry of Agriculture. Forty-eight male rats received 9L gliosarcoma and were randomly divided into (sorafenib) and untreated groups. At 1 day before treatment and at 1, 3, 5, and 8 days after the start of treatment, MR blood volume fractions were collected and oxygen saturation parameters were calculated. Evaluation of the correlation between living MR oximetry and assessment of hypoxia in vitro pimonidazole. Statistical analysis included paired and independent sample t-tests and Pearson’s parametric tests. Results MR oxygen saturation measurements of healthy tissue were consistent with those reported in the literature and were reproducible [mean (68.0 ± 6.5)% for all animals). There was a correlation between MR oxygen saturation measurement and immunohistochemical analysis (R2 = 0.81) in the area of hypoxic necrosis of the untreated tumor group. In the anti-angiogenic tumor group, the tumor MR saturation was lower than that in healthy tissues (P <0.001). Conclusions The results of this study show that the measurement of MR oxygen saturation is reproducible and can be used as an in vivo method for the detection of hypoxia in brain tumors and as a sensitive indicator of hypoxia after antiangiogenic therapy.