【摘 要】
：
目的 探讨N-乙酰半胱氨酸(NAC)对脓毒症肺损伤的作用及机制.方法 48只Balb/c小鼠随机分为对照组、模型组、NAC低剂量组、中剂量组、高剂量组和阳性药组(n=8).给予LPS前1h,腹腔注射药物干预研究.给予LPS 6 h后麻醉,收集肺泡灌洗液(BALF)和血清.苏木精-伊红染色检测肺损伤病理变化;ELISA检测BALF和血清中TNF-α、IL-6和IL-1β含量;Western blot检测组织中NLRP3、Caspase1和ASC蛋白水平;流式细胞术分析M2型巨噬细胞表型;商用试剂盒测量组织中
【机 构】
：
610051,成都医学院第二医附属医院核工业四一六医院重症医学科
论文部分内容阅读
目的 探讨N-乙酰半胱氨酸(NAC)对脓毒症肺损伤的作用及机制.方法 48只Balb/c小鼠随机分为对照组、模型组、NAC低剂量组、中剂量组、高剂量组和阳性药组(n=8).给予LPS前1h,腹腔注射药物干预研究.给予LPS 6 h后麻醉,收集肺泡灌洗液(BALF)和血清.苏木精-伊红染色检测肺损伤病理变化;ELISA检测BALF和血清中TNF-α、IL-6和IL-1β含量;Western blot检测组织中NLRP3、Caspase1和ASC蛋白水平;流式细胞术分析M2型巨噬细胞表型;商用试剂盒测量组织中MDA、MPO、SOD、GSH和ROS含量.结果 与模型组比较,NAC组小鼠肺损伤显著缓解,且BALF和血清中TNF-α、IL-6和IL-1β含量减少.NAC组较模型组小鼠BALF中M2型巨噬细胞表型增强,且NLRP3、Caspase1和ASC蛋白下调.NAC组小鼠肺组织中MDA、MPO和ROS含量减少,同时,SOD和GSH含量增加.结论 N-乙酰半胱氨酸对急性肺损伤具有保护作用,且可能与抑制ROS-NLRP3信号通路诱导巨噬细胞的M2型分化有关.
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