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用三个含有兔乳清酸性蛋白基因（ｒＷＡＰ）启动子，人ｏｈ基因（ｃＤＮＡ）及ｒＷＡＰ基因终止子的质粒经亚克隆构建了融合表达载体。用 Ｎｏｔｌ消化表达载体，回收包含上述三个表达元件且插入方向正确的片段（约 ７．５ ｋｂ）并进行显微注射。注射用的ＤＮＡ浓度为２ μｇ／ｍＬ，每ｍＬ注射液中ＤＮＡ的拷贝数约为２．４×１０~１１，每一枚原核期小鼠胚胎注射１－２ｐＬ。采用标准的显微注射方法，获得 ４８只后代小鼠。四周龄时剪尾并提取尾组织 ＤＮＡ，以人 ｏｂ基因 ｃＤＮＡ为探针，用 ＥｃｏＲＩ消化总组织ＤＮＡ，经Ｓｏｕｔｈｅｒｎ杂交确证其中两只母鼠为转人ｏｂ基因小鼠。在出生的后代小鼠中，转基因阳性率约为４％（２／４８）。
The fusion expression vector was constructed by subcloning three plasmids containing the rabbit whey acidic protein gene (rWAP) promoter, the human oh gene (cDNA) and the rWAP gene terminator. The expression vector was digested with Notl, the fragment containing the above three expression elements inserted in the correct orientation (about 7.5 kb) was recovered and subjected to microinjection. The concentration of DNA for injection is 2 μg / mL. The DNA copy number per mL of injection is approximately 2.4 × 10-11, and 1-2 pL is injected into each prokaryotic mouse embryo. Forty eight offspring mice were obtained using standard microinjection methods. At the age of 4 weeks, the tail tissue was tail-trimmed and the tail tissue DNA was extracted. The human ob gene cDNA was used as a probe to digest the total tissue DNA with EcoRI. Southern blotting confirmed that two of the female mice were ob gene mice. In offspring born offspring, the positive rate of transgene is about 4% (2/48).