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目的 研究消痰散结方对肿瘤细胞诱导的血管内皮细胞小管形成的影响 ;方法 用鼠尾中的弹性纤维蛋白制备凝胶用于血管形成实验 ,将内皮细胞团接种于凝胶中 ,将胃癌细胞接种于凝胶的上方进行两种细胞的复合培养 ,观察不同浓度的消痰散结方对胃癌细胞诱导的血管内皮细胞小管形成的影响。结果 消痰散结方 (5 0ug/mL、 1 0 0ug/mL、 2 0 0ug/mL)明显抑制胃癌MKN - 45细胞诱导的血管内皮细胞小管形成 ,与对照组相比有显著差异 (P <0 . 0 1 )。结论 消痰散结方抑制胃癌细胞诱导的内皮细胞小管形成可能是通过抑制癌细胞分泌血管形成因子 ,或抑制内皮细胞表达因子受体实现的
Objective To study the effect of Xiaotan Sanjie recipe on the formation of vascular endothelial cell tubules induced by tumor cells. METHODS: Elastin fibers from rat tail were used to prepare gels for angiogenesis experiments. Endothelial cell groups were inoculated into gels to treat gastric cancer. The cells were seeded on the top of the gel and cultured in vitro. The effects of different concentrations of Xiaotan Sanjie Fang on the formation of vascular endothelial cells in gastric cancer cells were observed. Results Xiaoyu Sanjie Formula (50 μg/mL,100 μg/mL,200 μg/mL) significantly inhibited the formation of vascular endothelial cells in gastric cancer MKN-45 cells, which was significantly different from the control group (P < 0 . 0 1 ). Conclusion Xiaoyu Sanjie Recipe inhibits the formation of endothelial cells in gastric cancer cells by inhibiting the secretion of angiogenic factors by cancer cells or inhibiting the expression of factor receptors in endothelial cells.