论文部分内容阅读
以铁丝为磁芯,盐酸小檗碱为目标分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂聚合成分子印迹搅拌棒,并对搅拌棒的制备条件和测定条件进行了选择。用该搅拌棒提取黄柏中的盐酸小檗碱,并用高效液相色谱法进行测定。盐酸小檗碱质量浓度在10~125 mg/L之间与峰面积A呈良好的线性关系,线性回归方程为:A=3.07×103ρ+1.27×106,相关系数r为0.9993,检出限为2.7 mg/L。加样回收率在94.5%~103.5%之间,RSD为3.3%。
The iron wire as the core, berberine as the target molecule, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the crosslinking agent polymerization into molecular imprinting stir bar, and stirring rod preparation conditions and determination Conditions have been selected. Berberine hydrochloride was extracted from the corkscrew with the stirring rod and assayed by high performance liquid chromatography. The linear regression equation of berberine hydrochloride was 10 ~ 125 mg / L and peak area A. The linear regression equation was: A = 3.07 × 103ρ + 1.27 × 106, the correlation coefficient r was 0.9993, the detection limit was 2.7 mg / L. The recoveries of samples ranged from 94.5% to 103.5% with a RSD of 3.3%.