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目的:对清脑复神液提取工艺进行再评价研究,为改进该制剂的生产工业提供实验依据。方法:采用HPLC测定黄芩苷含量,流动相甲醇-0.37%磷酸(47∶53),检测波长280 nm。以干膏量和黄芩苷提取量的综合评分为指标,通过正交试验考察溶媒用量、渗漉速度、乙醇体积分数对清脑复神液渗漉工艺的影响,利用紫外全波长扫描、近红外扫描和红外扫描比较不同提取液的理化性质。结果:最佳提取工艺为加8倍量40%乙醇于2.27 m L·min-1·kg-1渗漉;黄芩苷提取量分别为31.23 mg,干膏量21.64 g。渗漉法与浸渍法所得药液的干膏量及紫外全波长扫描、近红外扫描、红外扫描图谱均基本一致。结论:清脑复神液的渗漉工艺与原浸渍工艺提取的有效成分基本一致,可用渗漉工艺替代浸渍工艺。
OBJECTIVE: To re-evaluate the extraction process of Qingnao Fu Shen Ye to provide experimental basis for improving the production industry of this preparation. Methods: The content of baicalin was determined by HPLC. The mobile phase consisted of methanol - 0.37% phosphoric acid (47:53) and the detection wavelength was 280 nm. Based on the comprehensive score of the amount of dry cream and the amount of baicalin extracted, the effect of solvent amount, percolation rate and ethanol volume fraction on the percolation process of Qingnao Rejuvenation Fluid was investigated by orthogonal test. Comparison of physical and chemical properties of different extracts by scanning and infrared scanning. Results: The best extraction process was adding 8 times the amount of 40% ethanol in 2.27 m L · min-1 · kg-1 percolation; baicalin extract were 31.23 mg, dry cream volume 21.64 g. Percolation method and immersion method of the liquid obtained by the amount of paste and UV full-wavelength scanning, near infrared scanning, infrared scanning spectra are basically the same. CONCLUSION: The percolation process of Qingnao rejuvenation solution is basically the same as that of the active ingredients extracted from the original dipping process, and the dipping process can be replaced by the percolation process.