自 70年代末以来 ,许多研究者对茶树组织培养进行了研究 ,并取得了可喜的成果 ,以茶树营养器官和生殖器官作外植体培养均有成功报道。但云南大叶种茶树因其品种原因 ,茶多酚含量较高 ,外植体切段易氧化褐死。所以至今云南大叶种茶树组织培养成功的报道鲜见。本研究旨在了解大叶 Since the late 1970s, many researchers have conducted research on the tissue culture of tea trees and have achieved gratifying results. Success has been reported in the cultivation of vegetative organs and reproductive organs of tea trees as explants. However, the species of tea leaves of Yunnan big leaf species, tea polyphenols content is higher, explants cut easily oxidative brown death. So far, Yunnan big leaf tea tree tissue culture success stories rarely seen. This study aims to understand the big leaves