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目的:结合紫外光谱(UV-s)和惩罚岭型线性判别(PRLDA)模式识别方法建立短串联重复序列(STR)的基因分型方法。方法:以D16S539基因座的9-9,12-13两种基因型为研究对象,研究包含该多态性位点的DNA片段的聚合酶链反应(PCR)扩增条件和紫外检测条件,建立标准化扩增条件和检测条件,以此获得基因分型的标准物及其标准UV-s。以标准光谱为识别变量,建立了9-9与12-13基因型的PRLDA判别模型。结果:所建立的PRLDA模型有较大的类间距和较小的类内距,表明模型具有良好的稳健性,且预测样本均分布在校正样本集的范围之内,对预测样本也表现出了极强的判别能力。结论:该方法不需任何检测前处理,而只需一步PCR扩增和UV-s检测即可实现STR的基因分型,具有简单、快速、低成本等优点。
OBJECTIVE: To establish a genotyping method for short tandem repeat (STR) by UV-UV and PRLDA pattern recognition. Methods: The genotypes 9-9 and 12-13 of D16S539 locus were used as research objects to study the PCR amplification conditions and UV detection conditions of DNA fragments containing this polymorphic locus, and to establish Standardization of amplification conditions and detection conditions, in order to obtain the standard genotyping and its standard UV-s. Using the standard spectrum as the identification variable, the PRLDA discriminant model of 9-9 and 12-13 genotypes was established. Results: The established PRLDA model has larger inter-class spacing and smaller inter-class distance, indicating that the model has a good robustness. The predicted samples are all distributed within the range of the corrected sample set, and the predicted samples also show Strong discriminating ability. Conclusion: This method does not need any pretreatment, and only one-step PCR amplification and UV-s detection can be achieved STR genotyping, with the advantages of simple, rapid, low cost.