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目的:通过氯化锂-匹罗卡品反复致癎大鼠模型,探讨代谢型谷氨酸受体7(mGluR7)在癫癎发作过程中的作用。方法:大鼠分为正常对照组和模型组,用氯化锂-匹罗卡品制作癫癎模型,将模型组中致癎成功鼠分为反复刺激组和静止期组,致癎不成功作为不成功组。制作冰冻切片用免疫组化法检测4组大鼠海马CA1、CA3区及齿状回mGluR7光密度值。结果:与正常对照组相比,静止期组大鼠海马各区表达均减弱,以齿状回差别最明显,反复刺激组各区表达均明显增强,不成功组无明显改变。结论:mGluR7表达上调提示癫癎多次发作可能增强了其对谷氨酸释放的负反馈调节和摄取作用; mGluR7在静止期表达下调提示其可能有助于慢性期癫癎环路的形成。
OBJECTIVE: To investigate the role of metabotropic glutamate receptor 7 (mGluR7) in epileptic seizures induced by lithium-pilocarpine repeatedly. Methods: The rats were divided into normal control group and model group. Epilepsy model was made with lithium-pilocarpine, and the successful rats in model group were divided into repeated stimulation group and quiescent group. Unsuccessful group Frozen sections were made and the optical density of hippocampal CA1, CA3 and dentate gyrus mGluR7 were detected by immunohistochemistry. Results: Compared with normal control group, the expression of hippocampus in the quiescent group was weakened, the difference was most obvious in the dentate gyrus, and the expression in the repeated stimulation group was significantly increased, while in the unsuccessful group there was no obvious change. CONCLUSION: Upregulation of mGluR7 suggests that multiple episodes of epilepsy may enhance its negative feedback regulation and uptake of glutamate. Down-regulation of mGluR7 in quiescent phase suggests that mGluR7 may contribute to the formation of chronic epileptic loop.