论文部分内容阅读
用双重免疫染色包埋前免疫电镜法，在光镜和电镜水平观察了大鼠孤束核内降钙素基因相关肽神经元的分布及其神经末梢与儿茶酚胺能神经元之间的关系。降钙素基因相关肽阳性神经元呈棕色，主要分布于孤束核的连合亚核、内侧亚核以及背侧周边区。在孤束核的尾侧部、连合亚核的腹外侧部及背侧周边区观察到蓝绿色的酪氨酸羟化酶阳性神经元和棕色的降钙素基因相关肽阳性神经元和神经纤维，其中一些神经元内两者共存。电镜观察结果：酪氨酸羟化酶阳性神经元细胞质内有棒状或卵圆形的电子密度较高的吲哚-β-半乳糖苷酶反应产物，降钙素基因相关肽阳性神经末梢内见到电子密度较高的DAB反应产物以及清亮小泡和颗粒小泡。降钙素基因相关肽阳性神经末梢和酪氨酸羟化酶阳性神经元之间形成对称性轴一体突触。
Immunocytochemistry was used to observe the distribution of calcitonin gene-related peptide neurons in the nucleus tractus solitarius and the relationship between their nerve endings and catecholaminergic neurons. The calcitonin gene-related peptide positive neurons were brown and mainly distributed in the commissural subnucleus, the medial subnucleus and the dorsal peripheral area of the nucleus tractus solitarius. Cyan-tyrosine hydroxylase-positive neurons and brown calcitonin gene-related peptide-positive neurons and nerves were observed in the caudal part of the nucleus tractus solitarius, the ventrolateral part of the commissural subnucleus, and the dorsal peripheral part Fibers, some of which coexist in neurons. Electron microscopy results: tyrosine hydroxylase positive neurons within the cytoplasm of rod or oval electron density indole-β-galactosidase reaction product, calcitonin gene-related peptide positive nerve endings see To electron density DAB reaction products and clear vesicles and vesicles. Calcitonin gene-related peptide-positive nerve terminals and tyrosine hydroxylase-positive neurons form a symmetric axonal synapse.