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目的探讨谷胱甘肽Ｓ转移酶（ｇｌｕｔａｔｈｉｏｎｅＳｔｒａｎｓｆｅｒａｓｅ，ＧＳＴ）基因缺失与老年性白内障形成的关系。方法应用多聚酶链式反应（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＰＣＲ）方法对７７例老年性白内障患者及７６例健康对照者外周血细胞进行ＧＳＴＭ１基因检测，并对其中的２２例老年性白内障晶体上皮细胞进行ＧＳＴＭ１基因检测。结果白内障组ＧＳＴＭ１基因缺乏率为５３．２５％，对照组为４６．０５％，两组间差异无显著性（χ２＝０．７５０，Ｐ＞０．０５，ＯＲ＝０．７５）。２０例老年性白内障晶体上皮细胞内ＧＳＴＭ１基因检测与外周血细胞检测结果一致（Ｐ＜０．０１）。结论ＧＳＴＭ１基因缺失与老年性白内障的发生缺乏显著关系。
Objective To investigate the relationship between glutathione Stransferase (GST) gene deletion and age-related cataract formation. Methods GSTM1 gene was detected in 77 elderly cataract patients and 76 healthy controls using polymerase chain reaction (PCR). GSTM1 gene was detected in 22 elderly patients with cataractous lens epithelial cells. Results The incidence of GSTM1 gene deficiency was 53.25% in cataract patients and 46.05% in controls. There was no significant difference between the two groups (χ2 = 0.750, P> 0.05, OR = 0.75). The detection of GSTM1 gene in 20 senile cataractous lens epithelial cells was consistent with that of peripheral blood cells (P <0.01). Conclusion There is no significant relationship between GSTM1 gene deletion and senile cataract.