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1982年中国林科院从事松干蚧科研的同志在我省无锡等地松林调查到松材线虫.8月下旬,我处梅花山风景区生长健壮的黑松突然针叶枯萎,死亡8株.分别在树干、枝条检查1809平方厘米样方,发现树皮下、木质部表层有松褐天牛(Momochamusalernatus Hope)幼虫5头,坡面材小蠹(Xyleborus interjectus Blandford)、梢小蠹(Cryphalus sp.)等小蠹成、幼虫87头,马尾松角胫象(Shirahoshlzo patruclis(Voss))成、幼虫34头.未几日,卫桥、四方城以及5号、11号宾馆附近又陆续死亡10余株(按本处花旁附近1981年曾堆放因松毛虫为害致死的带皮原木数十万斤越夏越冬,毗连花房的上述地区松树上次期性害虫发生猖撅.)但附近马尾松已呈极度衰弱状态,未见成片死亡,而长势旺盛的黑松却频频迅速枯死.根据松褐大牛传播松材线虫和马尾松抗线虫、黑松不抗的文献记载,我们于10月14日用麻花钻在枯死黑松的离地面2尺处,每树钻取木屑约200毫升,共7株.将木屑置玻璃瓶中用蒸馏水中浸泡24小时后,吸去上清液,双层纱布滤去木屑,残液在1500转/分离心机上离心2分钟,用吸管吸去上清液,将残液置玻片上镜检,发现大量线虫.10月28日复请南京林产工业学院病理教研组朱熙樵、张九能两位讲师镜检,确认系松材线虫.
In 1982, the Chinese Academy of Forestry, engaged in research on pine and dried scales to investigate pine wood nematodes in the pine forests in Wuxi and other places of our province. In late August, a sudden growth of needles withered and died in the scenic spots of Meihua Mountain, Trunk and branches examination 1809 square centimeter quadrats, found under the bark, the surface of the xylem is the Monochamus (Momochamusalernatus Hope larvae) 5, the beetles (Xyleborus interjectus Blandford), small beetles (Cryphalus sp.) Beetles 87 larvae, Shirahoshlzo patruclis (Voss), and 34 larvae.A few days later, more than 10 plants died in the vicinity of Weiqiao, Sifang City and hotels 5 and 11 Near 1981, piles of logs with dead-skin-beds of hundreds of thousands of kilograms of winter-hardened winter pests that had died of detritus caused by the caterpillar were piled close to the flowers, and the pests in the pine trees in the above-mentioned areas adjoining the flower parcel were rampant.) However, No dead, but the vigorous growth of black pine is often rapidly dead.According to the loose brown cattle disseminated pine wood nematode and masson pine nematodes, black pine anti-literatures, we on October 14 with a twist drill Withered black pine from the ground 2 feet , Each tree to get about 200 milliliters of sawdust, a total of 7. Sawdust in glass bottles soaked in distilled water for 24 hours, the supernatant was aspirated, double gauze filtered to remove sawdust, raffinate 1500 centrifuge / centrifuge Centrifuged for 2 minutes, pipette sucked the supernatant, the residue on the slide glass microscopy and found a large number of nematodes.10 Oct. 28, please reply Nanjing Institute of Pathology, Institute of Pathology Professor Zhu Xiqiao, Zhang nine to mirror the two lecturers, confirmed Department of pine Nematodes.