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目的:评价前列腺特异性抗原(PSA)及PSAmRNA对前列腺癌的诊断意义。方法:选取前列腺癌患者28例作为受试对象,选取前列腺增生患者30例作为对照组,采用ELISA法检测血清PSA水平,采用反转录聚合酶链反应检测其mRNA的表达。结果:前列腺增生患者总PSA<10 ng/ml,而前列腺癌患者总PSA>10 ng/ml。当PSA在诊断灰区4~10 ng/ml之间时,PSAmRNA与金标准相比,无统计学意义。结论:检测PSA的同时进行PSAmRNA的检测,有助于前列腺癌的诊断。
Objective: To evaluate the diagnostic value of prostate-specific antigen (PSA) and PSA mRNA in prostate cancer. Methods: Twenty-eight prostate cancer patients were selected as experimental subjects. Thirty patients with benign prostatic hyperplasia were selected as control group. Serum PSA level was measured by ELISA. The mRNA expression was detected by reverse transcription-polymerase chain reaction. Results: The total PSA in prostate hyperplasia was <10 ng / ml, while the total PSA in prostate cancer was> 10 ng / ml. PSA PSA was not statistically significant when compared with the gold standard when the PSA was between 4 and 10 ng / ml in the diagnostic gray zone. Conclusion: The detection of PSA mRNA simultaneously with PSA mRNA is helpful for the diagnosis of prostate cancer.